Coding
Part:BBa_K2220018:Design
Designed by: Nan Rong Group: iGEM17_BNU-China (2017-10-22)
Display mCherry protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 325
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 388
Illegal XhoI site found at 1102 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1171
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1153
Design Notes
We have done the codon optimization.
Source
Aga2 comes from pYD1 plasmid.